https://eln.excellgen.com/index.php?action=history&feed=atom&title=1967-pCMV5-RSV-Gag-NLS-Cre-WPRE-oriP 1967-pCMV5-RSV-Gag-NLS-Cre-WPRE-oriP - Revision history 2026-10-18T22:59:55Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1967-pCMV5-RSV-Gag-NLS-Cre-WPRE-oriP&diff=11418&oldid=prev Paul.xu: Created page with "== '''Cloning of 1967-pCMV5-RSV-Gag-NLS-Cre-WPRE-oriP''' == '''Purpose:''' Cloning of 1967-pCMV5-RSV-Gag-NLS-Cre-WPRE-oriP '''Date: '''2014-3-5---2014-3-7 '''Notebook:0''' ..." 2014-03-07T21:13:23Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1967-pCMV5-RSV-Gag-NLS-Cre-WPRE-oriP&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1967-pCMV5-RSV-Gag-NLS-Cre-WPRE-oriP &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-3-5---2014-3-7 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1967-pCMV5-RSV-Gag-NLS-Cre-WPRE-oriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1967-pCMV5-RSV-Gag-NLS-Cre-WPRE-oriP<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-3-5---2014-3-7<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1967&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1967_2081clone_3-5-2014.JPG]]<br /> <br /> {| width=&quot;322&quot; height=&quot;128&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP&lt;br&gt;<br /> | 2076 pCMV5-RSV-Gag-NLS-Flp-WPRE-oriP&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | EcoR1 + Xba1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-3-7<br /> <br /> Make miniprep and digest with BamH1-Kpn1&lt;br&gt;<br /> <br /> [[Image:1967digest_3-7-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; [[Category:Cloning]]</div> Paul.xu