https://eln.excellgen.com/index.php?action=history&feed=atom&title=1968_pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP - Revision history 2026-10-18T22:24:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1968_pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP&diff=10818&oldid=prev Paul.xu: Created page with "== '''Cloning of 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriPP''' == '''Purpose:''' Cloning of 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP '''Date: '''2013-12-15--- '''Notebook:0''' '''..." 2013-12-18T21:14:40Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriPP&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-15--- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriPP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-15--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1968&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> subclone NLS-Cre from 1400 pCMV5-Gag-NLS-Cre-WPRE-oriP to 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP or 1970 pCMV5-Gag-p2b-TagGFP-WPRE-oriP <br /> <br /> USING EcoR1+Eag1 <br /> <br /> [[Image:DIGEST_FOR_1987_1988_1968.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-12-5 <br /> <br /> Make miniprep, digest with EcoR1 +EagI&lt;br&gt; <br /> <br /> [[Image:1968digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu