https://eln.excellgen.com/index.php?action=history&feed=atom&title=1969_pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP - Revision history 2026-10-18T13:43:58Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1969_pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP&diff=10740&oldid=prev Paul.xu: Created page with "== '''Cloning of 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP''' == '''Purpose:''' Cloning of 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP '''Date: '''2013-12-4--- '''Notebook:0''' '''Pa..." 2013-12-06T19:26:17Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-4--- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Pa...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-4--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1969&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR out Gag from 1445 pCMV5-Gag-NLS-Flp-WPRE-oriP using Gag-BamH1-F+HIV-Gag-Kpn1-EcoR1-R <br /> <br /> [[Image:1968_1969_1970PCR.JPG‎ ]]&lt;br&gt;<br /> <br /> digest with EcoR1 + BamH1 and clone back into 1445 pCMV5-Gag-NLS-Flp-WPRE-oriP <br /> <br /> [[Image:DIGEST FOR 1968 1969 1970 1941.JPG]]<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-12-5 <br /> <br /> Make miniprep, digest with EcoR1 + BamH1&lt;br&gt; <br /> <br /> [[Image:1969_1970digest.JPG]]&lt;br&gt; <br /> <br /> send 1969-1 to sequence with HIV-Gag-seq-F&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu