https://eln.excellgen.com/index.php?action=history&feed=atom&title=1972-pCMV5-Tat-Cre-PDGFR
1972-pCMV5-Tat-Cre-PDGFR - Revision history
2026-10-18T11:22:56Z
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Paul.xu at 15:51, 28 January 2014
2014-01-28T15:51:04Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 1972-pCMV5-Tat-Cre-PDGFR''' ==<br />
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'''Purpose:''' Cloning of 1972-pCMV5-Tat-Cre-PDGFR <br />
<br />
'''Date: '''2014-1-14---2014-1-23 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1972''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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pcr1 the same with 1961pcr1<br><br />
<br />
pcr2 PCR iCre from 1520 pDIRE<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; NLS-iCre-HindIII-F iCre-Kpn1-R<br><br />
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[[Image:718_1972_1973_1974_1997_2012pcr_1-2-2014.JPG]]<br><br />
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PCR :pcr1+pcr2<br><br />
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[[Image:715_718_1315_1946_1972_1984_2012PCR_1-6-2014.JPG]]<br><br />
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[[Image:1322_1972clone_1-15-2014.jpg]]<br><br />
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{| width="293" height="116" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| lane <br><br />
| 3<br><br />
| 4<br><br />
|-<br />
| <br><br />
| pcr <br><br />
| 1971 pCMV5-hIgG-Lc-FN3K-PDGFR-WPRE-OriP<br><br />
|-<br />
| <br><br />
| colspan="2" | Nhe1 + Kpn1<br><br />
|}<br />
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<br><br />
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=== Colony Screening ===<br />
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Date: 204-1-23 <br />
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Make miniprep and digest with Hind111 + Kpn1<br />
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[[Image:1972_1322_2024digest_1-23-2014.JPG]]<br> <br />
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<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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<br> <br />
<br />
[[Category:Cloning]]</div>
Paul.xu