https://eln.excellgen.com/index.php?action=history&feed=atom&title=1981-pCMV5-Tat-Flp-PDGFR 1981-pCMV5-Tat-Flp-PDGFR - Revision history 2026-10-18T07:07:22Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1981-pCMV5-Tat-Flp-PDGFR&diff=11296&oldid=prev Paul.xu: Created page with "== '''Cloning of 1981-pCMV5-Tat-Flp-PDGFR''' == '''Purpose:''' Cloning of 1981-pCMV5-Tat-Flp-PDGFR '''Date: '''2014-2-18--2014-2-20 '''Notebook:0''' '''Page:''' '''Clone..." 2014-02-21T16:45:42Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1981-pCMV5-Tat-Flp-PDGFR&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1981-pCMV5-Tat-Flp-PDGFR &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-18--2014-2-20 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1981-pCMV5-Tat-Flp-PDGFR&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1981-pCMV5-Tat-Flp-PDGFR<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-18--2014-2-20 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1981&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1954_1964_1965_1966_1981_2019pcr_1-16-2014.jpg]]&lt;br&gt; <br /> <br /> pcr1:PCR His-Tat-NLS from 1860 pT7A3-yFLP<br /> <br /> 6His-Nhe1-F &lt;br&gt;NLS-HindIII-R&lt;br&gt;<br /> <br /> pcr2:PCR Flpo from 1520 pDIRE&lt;br&gt;<br /> <br /> NLS-Flpo-HindIII-F &lt;br&gt;<br /> <br /> Flp-nostop-Kpn1-R&lt;br&gt;<br /> <br /> [[Image:725_1981pcr_1-17-2014.jpg]]&lt;br&gt;<br /> <br /> PCR:pcr1+pcr2&lt;br&gt;<br /> <br /> 6His-Nhe1-F&lt;br&gt;Flp-nostop-Kpn1-R &lt;br&gt;<br /> <br /> [[Image:1146_1981_2012_2056clone_2-18-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1972,1972 pCMV5-Tat-Cre-PDGFR&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nhe1 + Kpn1&lt;br&gt;<br /> |}<br /> <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-20<br /> <br /> Make miniprep and digest with&amp;nbsp; Hind1111 + Kpn1&lt;br&gt;<br /> <br /> [[Image:1146_1981digest_2-20-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu