https://eln.excellgen.com/index.php?action=history&feed=atom&title=1983-pEC-SM2 1983-pEC-SM2 - Revision history 2026-10-18T17:37:01Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1983-pEC-SM2&diff=10856&oldid=prev Paul.xu: Created page with "== '''Cloning of 1983-pEC-SM2''' == '''Purpose:''' Cloning of 1983-pEC-SM2 '''Date: '''2013-12-23---2013-12-26 '''Notebook:0''' '''Page:''' '''Clone ID:1983''' '''Exper..." 2013-12-26T17:50:14Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1983-pEC-SM2&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1983-pEC-SM2 &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-23---2013-12-26 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1983&#039;&#039;&#039; &#039;&#039;&#039;Exper...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1983-pEC-SM2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1983-pEC-SM2<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-23---2013-12-26<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1983&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1948+1983pcr_12-23-2013.JPG]]&lt;br&gt; <br /> <br /> gene synthesis&lt;br&gt;<br /> <br /> Sm2-1&amp;nbsp; to Sm2-18&lt;br&gt;<br /> <br /> [[Image:1948+1983clone_12-23-2013.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;264&quot; height=&quot;67&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr &lt;br&gt;<br /> | 1454 pEC&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | EcoR1 + HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-12-26<br /> <br /> Make miniprep and digest with&amp;nbsp;&amp;nbsp; EcoR1 + HindIII<br /> <br /> [[Image:1983digest_12-26-2013.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu