https://eln.excellgen.com/index.php?action=history&feed=atom&title=1991-pT7A3E-HNH 2026-10-18T12:09:00Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1991-pT7A3E-HNH&diff=10942&oldid=prev 2014-01-14T21:10:44Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1991-pT7A3E-HNH&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1991-pT7A3E-HNH <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-30---2014-1-2 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1991&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1943_1991pcr_12-30-2013.JPG]]&lt;br&gt;<br /> <br /> colicin-E9-HNH-1 to colicin-E9-HNH-4&lt;br&gt;<br /> <br /> [[Image:1315_1943_1946_1984_1991clone_12-31-2013.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | 9&lt;br&gt;<br /> | 10&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR &lt;br&gt;<br /> | 1892 pT7A3E&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | HindIII + Sal1&lt;br&gt;<br /> | HindIII + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-2 <br /> <br /> Make miniprep and digest with HindIII + Sma1&lt;br&gt;<br /> <br /> [[Image:1948_1991digest_1-2-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu