https://eln.excellgen.com/index.php?action=history&feed=atom&title=1994_pT7A3E-Exo-III 1994 pT7A3E-Exo-III - Revision history 2026-10-18T22:23:44Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1994_pT7A3E-Exo-III&diff=10989&oldid=prev Paul.xu at 18:52, 17 January 2014 2014-01-17T18:52:58Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1994 pT7A3E-Exo-III&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1994 pT7A3E-Exo-III <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1994&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> subclone Ecoli exonuclease III from 1136 to 1892(-4) pT7A3E,using NdeI+XhoI <br /> <br /> [[Image:DIGEST FOR 1947 1994 1998 2000 1944 1950.JPG]]&lt;br&gt; <br /> <br /> [[Image:Digest vectors for 1886 1887 1938 1939 1947 1993 1994 1998 2000 1535 1588.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> Make miniprep, digest with NdeI+XhoI &lt;br&gt; <br /> <br /> [[Image:1993 1994 1998digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu