https://eln.excellgen.com/index.php?action=history&feed=atom&title=1997-pL6H 1997-pL6H - Revision history 2026-10-18T20:10:21Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1997-pL6H&diff=11023&oldid=prev Paul.xu: Created page with "== '''Cloning of 1997-pL6H''' == '''Purpose:''' Cloning of 1997-pL6H '''Date: '''2014-1--10---2014-1-15 '''Notebook:0''' '''Page:''' '''Clone ID:1997''' '''Experimenter..." 2014-01-17T21:28:09Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1997-pL6H&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1997-pL6H &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1--10---2014-1-15 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1997&#039;&#039;&#039; &#039;&#039;&#039;Experimenter...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1997-pL6H&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1997-pL6H<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1--10---2014-1-15<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1997&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:718_1972_1973_1974_1997_2012pcr_1-2-2014.JPG]]&lt;br&gt; <br /> <br /> PCR lambda pL promoter from 1712 pBV220-hINFA2 &lt;br&gt;PL-BglII-F &lt;br&gt;PL-Nde1-R&lt;br&gt;<br /> <br /> [[Image:1997_2012_2013_2024clone_1-14-2014.jpg]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1984 pL42&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | BglII + Nde1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-16<br /> <br /> Make miniprep and digest with&amp;nbsp; BglII + Nco1&lt;br&gt;<br /> <br /> [[Image:1997_2012digest_1-16-2014.jpg]]&lt;br&gt; <br /> <br /> c:vector&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu