https://eln.excellgen.com/index.php?action=history&feed=atom&title=2000_pT7A3-8H-E_coli_Endo_IV 2000 pT7A3-8H-E coli Endo IV - Revision history 2026-10-18T21:32:36Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2000_pT7A3-8H-E_coli_Endo_IV&diff=11290&oldid=prev Paul.xu at 15:15, 21 February 2014 2014-02-21T15:15:15Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2000 pT7A3-8H-E coli Endo IV&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2000 pT7A3-8H-E coli Endo IV <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2000&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> subclone E coli Endonuclease IV from 1593 to 1725 pT7A3-8H,using NdeI+XhoI <br /> <br /> [[Image:Digest vectors for 1886 1887 1938 1939 1947 1993 1994 1998 2000 1535 1588.JPG]] <br /> <br /> [[Image:DIGEST FOR 1947 1994 1998 2000 1944 1950.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> Make miniprep, digest with NdeI+XhoI &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2000digest.JPG]] <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu