https://eln.excellgen.com/index.php?action=history&feed=atom&title=2012-pL42-Dnase1 2012-pL42-Dnase1 - Revision history 2026-10-18T16:39:55Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2012-pL42-Dnase1&diff=12769&oldid=prev Excellgen at 18:47, 8 August 2014 2014-08-08T18:47:53Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2012-pL42-Dnase1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2012-pL42-Dnase1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-13---2014-1-16 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2012&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:718 1972 1973 1974 1997 2012pcr 1-2-2014.JPG]]&lt;br&gt; <br /> <br /> PCR DNase 1 from 202 using &lt;br&gt;DNAse1-Nde1-F &lt;br&gt;DNAse1-22&lt;br&gt; <br /> <br /> [[Image:1997 2012 2013 2024clone 1-14-2014.jpg]] <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pcr&lt;br&gt; <br /> | 1984 pL42&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 +Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-17 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1997 2012digest 1-16-2014.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Cloning of 2012-pL42-Dnase1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2012-pL42-Dnase1&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2012&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR DNase 1 from 202 using &lt;br&gt;DNAse1-Nde1-F atcgac cat atg ctg aagatcgcagctttcaacatc&lt;br&gt;DNAse1-22 (5/3/2011)&lt;br&gt;(CACTAAGGTACCCTCGAGAATCC)&lt;br&gt;GGATTCTCGAGGGTACCTTAGTG <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; clone into 1984 pL42 Nde1 +Xho1&lt;br&gt; <br /> <br /> [[Image:Digest for 2249 2012 2049 2197.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_7 <br /> <br /> Make miniprep, use Nde1 +Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:1577 1578 2012 2049digest1.JPG]]&lt;br&gt; <br /> <br /> use PstI to verify&lt;br&gt; <br /> <br /> [[Image:2238 1577 1578 2186 2272 2012digest.JPG]] <br /> <br /> sent 2012-2 to sequence, missing one g&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 2012-pL42-Dnase1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2012-pL42-Dnase1&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2012&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR DNase 1 from 2012-2 using &lt;br&gt;DNAse1-Nde1-F atcgac cat atg ctg aagatcgcagctttcaacatc&lt;br&gt;DNAse1-22 (5/3/2011)&lt;br&gt;(CACTAAGGTACCCTCGAGAATCC)&lt;br&gt;GGATTCTCGAGGGTACCTTAGTG <br /> <br /> [[Image:2297_2012fixpcr.JPG]]&lt;br&gt; clone into 1984 pL42 Nde1 +Xho1&lt;br&gt; <br /> <br /> [[Image:Digest_for2012fix_2166.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_7 <br /> <br /> Make miniprep, use Nde1 +Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2012FIX_2303_1578_2166digest.JPG]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen