https://eln.excellgen.com/index.php?action=history&feed=atom&title=2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP - Revision history 2026-10-18T07:54:55Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP&diff=11128&oldid=prev Paul.xu: Created page with "== '''Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP''' == '''Purpose:''' Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP '''Date: '''2014-1-27---2014-1-2..." 2014-01-30T16:02:05Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-27---2014-1-2...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-27---2014-1-29<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2013&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2013pcr_1-27-2014.JPG]]<br /> <br /> PCR FRT-TurboRFP-ODC-2Stop-FRT-TurboGFP from 1922 pLVX-Kozak-FRT-TurboRFP-ODC-2Stop-FRT-TurboGFP&lt;br&gt;Nhe1-BamH1-Kozak-FRT-F&lt;br&gt;TurboGFP-HindIII-Sal1-R&lt;br&gt;<br /> <br /> [[Image:1965_2013clone_1-27-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;354&quot; height=&quot;116&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1522 CMV-Brainbow-2.1-R&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | Nhe1 + Sal1&lt;br&gt;<br /> | Nhe1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-29<br /> <br /> Make miniprep and digest with HindIII + BamH1&lt;br&gt;<br /> <br /> [[Image:1965_2013digest_1-29-2014.JPG]]&lt;br&gt; <br /> <br /> c:vector-1522 CMV-Brainbow-2.1-R&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu