https://eln.excellgen.com/index.php?action=history&feed=atom&title=2016-pLC6H-Sso-SSB 2016-pLC6H-Sso-SSB - Revision history 2026-10-18T09:25:19Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2016-pLC6H-Sso-SSB&diff=11241&oldid=prev Paul.xu: Created page with "== '''Cloning of 2016-pLC6H-Sso-SSB''' == '''Purpose:''' Cloning of 2016-pLC6H-Sso-SSB '''Date: '''2014-2-12---2014-2-14 '''Notebook:0''' '''Page:''' '''Clone ID:2016''' ..." 2014-02-14T21:36:46Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2016-pLC6H-Sso-SSB&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2016-pLC6H-Sso-SSB &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-12---2014-2-14 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2016&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2016-pLC6H-Sso-SSB&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2016-pLC6H-Sso-SSB<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-12---2014-2-14<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2016&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1142_2016_2033pcr_1-28-2014.JPG]]<br /> <br /> PCR from Sulfolobus solfataricus genomic DNA, ATCC box&lt;br&gt;Sso-SSB-Nde1-F&lt;br&gt;Sso-SSB-Xho1-R&lt;br&gt;<br /> <br /> [[Image:1114_1142_1940_2016clone_2-12-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;243&quot; height=&quot;64&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 7&lt;br&gt;<br /> | 8&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 2030 pLC6H-sfGFP&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + Xho1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-14<br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1940_2016digest_2-14-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu