https://eln.excellgen.com/index.php?action=history&feed=atom&title=2019-pT7A3-Endo-III 2019-pT7A3-Endo-III - Revision history 2026-10-18T19:25:09Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2019-pT7A3-Endo-III&diff=11145&oldid=prev Paul.xu at 15:32, 4 February 2014 2014-02-04T15:32:59Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2019-pT7A3-Endo-III&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2019-pT7A3-Endo-III <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-16--2014-1-24 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2019&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1954 1964 1965 1966 1981 2019pcr 1-16-2014.jpg]]&lt;br&gt; <br /> <br /> pcr from bl21(de3)&lt;br&gt; <br /> <br /> ecoli-Endo-III-Nde1-F&lt;br&gt;ecoli-Endo-III-Xho1-R&lt;br&gt; <br /> <br /> [[Image:1954 1964 1966 1945 1979 2019clone 1-16-2014.jpg]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane &lt;br&gt; <br /> | 9&lt;br&gt; <br /> | 10&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pcr&lt;br&gt; <br /> | 1885 pT7A3-HC&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-24 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1979 2019digest 1-24-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Subcloning of 2019-pT7A3E-Endo-III&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Subcloning of 2019-pT7A3E-Endo-III<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-28------2014-1-31 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2019&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2011_2019_2013clone_1-29-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | LANE&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 2019-1&lt;br&gt;<br /> | 1892 pT7A3E&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + Xho1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-31<br /> <br /> Make miniprep and digest with&amp;nbsp; Nde1 + Xho1 <br /> <br /> [[Image:2010_2011_2019digest1-31-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu