https://eln.excellgen.com/index.php?action=history&feed=atom&title=2021-pT7A3E-6H-HC 2026-10-18T14:13:18Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2021-pT7A3E-6H-HC&diff=10977&oldid=prev 2014-01-15T14:52:11Z

<p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2021-pT7A3E-6H-HC&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2021-pT7A3E-6H-HC &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-10---2014-1-13 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2021&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2021-pT7A3E-6H-HC&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2021-pT7A3E-6H-HC<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-10---2014-1-13<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2021&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1973_1979_2003_2021_2024clone_1-10-2014.JPG]]<br /> <br /> digest 1885 pT7A3-HC(vector) with Xba1 + Nde1,&lt;br&gt;ligate to annealed oligos:&lt;br&gt;Xba1-6H-Nde1-T &lt;br&gt;Xba1-6H-Nde1-B&lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-13<br /> <br /> Make miniprep and digest with Nco1 + EcoRV,linearized<br /> <br /> [[Image:2021digest_1-13-2014.jpg]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div>

Paul.xu