https://eln.excellgen.com/index.php?action=history&feed=atom&title=2026_pT7A3E-T4-Ligase-HI-6H 2026 pT7A3E-T4-Ligase-HI-6H - Revision history 2026-10-18T15:42:11Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2026_pT7A3E-T4-Ligase-HI-6H&diff=11005&oldid=prev Paul.xu: Created page with "== '''Cloning of 2026 pT7A3E-T4-Ligase-HI-6H''' == '''Purpose:''' Cloning of 2026 pT7A3E-T4-Ligase-HI-6H'''==''' '''Date: '''2014-1---- '''Notebook:0''' '''Page:''' '''..." 2014-01-17T19:16:54Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2026 pT7A3E-T4-Ligase-HI-6H&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2026 pT7A3E-T4-Ligase-HI-6H&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2026 pT7A3E-T4-Ligase-HI-6H&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2026 pT7A3E-T4-Ligase-HI-6H&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2026&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> subclone T4-Ligase from 1927 to&amp;nbsp; 1923 pT7A3E-HI ,using Nde1 + XhoI <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Digest for 2025 2026 2027 2032 2031.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> Make miniprep, digest with NdeI+XhoI &lt;br&gt; <br /> <br /> [[Image:1992 2025 2026DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu