https://eln.excellgen.com/index.php?action=history&feed=atom&title=2028_pT7A3-Fast-Kfu
2028 pT7A3-Fast-Kfu - Revision history
2026-10-18T20:10:50Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=2028_pT7A3-Fast-Kfu&diff=11273&oldid=prev
Paul.xu at 20:42, 20 February 2014
2014-02-20T20:42:08Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 2028 pT7A3-Fast-Kfu''' ==<br />
<br />
'''Purpose:''' Cloning of2028 pT7A3-Fast-Kfu'''==''' <br />
<br />
'''Date: '''2014-2---- <br />
<br />
'''Notebook:0''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:2028''' <br />
<br />
'''Experimenters:'''&nbsp; shanyi <br />
<br />
== '''Results''' ==<br />
<br />
=== CLONING ===<br />
<br />
PCR <br />
<br />
1<br>Fast-Kfu-SacI-F C10 atggaaggagctcgaagaaaag<br>fast-Kfu-F591L-R C9 cttcttcgtgacgaaCAGgccgcgtttgtag<br>2.<br>fast-Kfu-F591L-F C8 ctacaaacgcggcCTGttcgtcacgaagaag<br>Fast-Kfu-HindIII-R C11 ttagcaaagcttcaagaaccctc<br />
<br />
[[Image:2028_2062firstpcr_2068pcr.JPG]]<br><br />
<br />
<br>Join 2 fragments by PCR<br>Fast-Kfu-SacI-F C10 atggaaggagctcgaagaaaag<br>Fast-Kfu-HindIII-R C11 ttagcaaagcttcaagaaccctc<br />
<br />
[[Image:2062_2028pcr.JPG]]<br><br />
<br />
digest and clone back into SacI + HindIII digested 1995 pT3A3-Kfu-sso7d <br>[[Image:DIGEST_FOR_2063_2028.JPG]]SacI + HindIII<br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2014<br />
<br />
Make miniprep, digest with SacI + HindIII<br> <br />
<br />
[[Image:2028_2038_2059_2061DIGEST.JPG]]<br> <br />
<br />
<br> <br />
<br />
<br> <br />
<br />
<br> <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
*<br><br />
<br />
== '''Conclusion''' ==<br />
<br />
[[Category:Cloning]]</div>
Paul.xu