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2030-pLC6H-sfGFP - Revision history
2026-10-18T11:52:05Z
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Paul.xu at 19:52, 10 February 2014
2014-02-10T19:52:42Z
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<p><b>New page</b></p><div>== '''Cloning of 2030-pLC6H-sfGFP''' ==<br />
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'''Purpose:''' Cloning of 2030-pLC6H-sfGFP <br />
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'''Date: '''2014-2-4---2014-2-7 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:2030''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:2029 2039 2045 2047 2067 2069pcr 2-5-2014.JPG]] <br />
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PCR using oligos<br>sfGFP-EcoR1-F <br />
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sfGFP-Not1-Xho1-6H-Pst1-R<br> <br />
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[[Image:2029 2030 2047 2067clone 2-5-2014.JPG]]<br> <br />
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{| width="259" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| lane<br> <br />
| 3<br> <br />
| 4<br><br />
|-<br />
| <br> <br />
| pcr<br> <br />
| 1712, pLambda-hINFA2<br><br />
|-<br />
| <br> <br />
| colspan="2" | EcoR1 + Pst1<br><br />
|}<br />
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<br> <br />
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=== Colony Screening ===<br />
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Date: 2014-2-7 <br />
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Make miniprep and digest with EcoR1 + Pst1<br> <br />
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[[Image:2029 2030digest 2-7-2014.JPG]]<br> <br />
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<br><br />
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2014-2-10<br><br />
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digest with Bgl11+Xho1<br><br />
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[[Image:2029+2030digest_2-10-2014.JPG]]<br />
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&lt;br&gt; <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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<br> <br />
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[[Category:Cloning]]</div>
Paul.xu