https://eln.excellgen.com/index.php?action=history&feed=atom&title=2031_2032 2026-10-18T05:42:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2031_2032&diff=16627&oldid=prev 2017-11-19T16:35:09Z

<p></p> <p><b>New page</b></p><div>== Protein Expression ==<br /> <br /> 2014/1/14<br /> <br /> &lt;span style=&quot;color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;&quot;&gt;PageRuler Plus 10, 15, 25(red), 35, 55, 70(red), 100, 130, 250&lt;/span&gt;&lt;br/&gt;Paul<br /> <br /> [[File:2031 2032 expression 1 14 2014.jpg|RTENOTITLE]]<br /> <br /> {| width=&quot;832&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br/&gt;<br /> | Name&lt;br/&gt;<br /> | Protein Name&lt;br/&gt;<br /> | Clone ID&lt;br/&gt;<br /> | Host&lt;br/&gt;<br /> | Induction&lt;br/&gt;<br /> | Purification&lt;br/&gt;<br /> | pI/Mw&lt;br/&gt;<br /> |-<br /> | 1&lt;br/&gt;<br /> | pCold-Tat-yFLP&lt;br/&gt;<br /> | Tat-yFLP&lt;br/&gt;<br /> | 2031&lt;br/&gt;<br /> | <br /> 5 ml,<br /> <br /> Nico21(DE3)pRare2pLysS<br /> <br /> | 1 mM IPTG&amp;nbsp; RT o/n&lt;br/&gt;<br /> | <br /> pellet,&amp;nbsp;dissolved in 8M Urea (Insoluble fraction)<br /> <br /> Ni beads<br /> <br /> | <br /> 9.76 / 52.8<br /> <br /> |-<br /> | 2&lt;br/&gt;<br /> | pCold-Tat-yFLP&lt;br/&gt;<br /> | Tat-yFLP&lt;br/&gt;<br /> | 2031&lt;br/&gt;<br /> | <br /> &amp;nbsp;5 ml,<br /> <br /> Nico21(DE3)pRare2pLysS<br /> <br /> | 1 mM IPTG RT o/n&lt;br/&gt;<br /> | <br /> Soluble fraction of cell lysate<br /> <br /> Ni beads<br /> <br /> | <br /> 9.76 / 52.8<br /> <br /> |-<br /> | 3&lt;br/&gt;<br /> | pCold-Tat-yFLP&lt;br/&gt;<br /> | Tat-yFLP&lt;br/&gt;<br /> | 2031&lt;br/&gt;<br /> | <br /> 5 ml,<br /> <br /> Nico21(DE3)pRare2pLysS<br /> <br /> | 1 mM IPTG RT o/n&lt;br/&gt;<br /> | <br /> pellet, dissolved in 8M Urea (Insoluble fraction)<br /> <br /> Ni beads<br /> <br /> | <br /> 9.76 / 52.8<br /> <br /> |-<br /> | 4&lt;br/&gt;<br /> | pCold-Tat-yFLP&lt;br/&gt;<br /> | Tat-yFLP&lt;br/&gt;<br /> | 2031&lt;br/&gt;<br /> | <br /> 5 ml,<br /> <br /> Nico21(DE3)pRare2pLysS<br /> <br /> | 1 mM IPTG RT o/n&lt;br/&gt;<br /> | <br /> Soluble fraction of cell lysate<br /> <br /> Ni Magnetic beads<br /> <br /> | <br /> 9.76 / 52.8<br /> <br /> |-<br /> | 5&lt;br/&gt;<br /> | PageRuler Plus Prestained protein ladder&lt;br/&gt;<br /> | colspan=&quot;6&quot; | &lt;br/&gt;<br /> |-<br /> | 6&lt;br/&gt;<br /> | pCold-Tat-Flpo&lt;br/&gt;<br /> | Tat-Flpo&lt;br/&gt;<br /> | 2032&lt;br/&gt;<br /> | 5 ml,<br /> Nico21(DE3)pRare2pLysS<br /> <br /> | 1 mM IPTG RT o/n&lt;br/&gt;<br /> | <br /> pellet, dissolved in 8M Urea (Insoluble fraction)<br /> <br /> Ni beads<br /> <br /> | <br /> 9.76 / 52.8<br /> <br /> |-<br /> | 7&lt;br/&gt;<br /> | pCold-Tat-Flpo&lt;br/&gt;<br /> | Tat-Flpo&lt;br/&gt;<br /> | 2032&lt;br/&gt;<br /> | &amp;nbsp; 5 ml,<br /> Nico21(DE3)pRare2pLysS<br /> <br /> <br /> <br /> | 1 mM IPTG RT o/n&lt;br/&gt;<br /> | <br /> Soluble fraction of cell lysate<br /> <br /> Ni beads<br /> <br /> | <br /> 9.76 / 52.8<br /> <br /> |-<br /> | 8&lt;br/&gt;<br /> | pCold-Tat-Flpo&lt;br/&gt;<br /> | Tat-Flpo&lt;br/&gt;<br /> | 2032&lt;br/&gt;<br /> | &amp;nbsp;5 ml,<br /> Nico21(DE3)pRare2pLysS<br /> <br /> <br /> <br /> | 1 mM IPTG RT o/n&lt;br/&gt;<br /> | pellet, dissolved in 8M Urea (Insoluble fraction)&lt;br/&gt;Ni beads&lt;br/&gt;<br /> | <br /> 9.76 / 52.8<br /> <br /> |-<br /> | 9&lt;br/&gt;<br /> | pCold-Tat-Flpo&lt;br/&gt;<br /> | Tat-Flpo&lt;br/&gt;<br /> | 2032&lt;br/&gt;<br /> | &amp;nbsp; 5 ml,<br /> Nico21(DE3)pRare2pLysS<br /> <br /> <br /> <br /> | 1 mM IPTG RT o/n&lt;br/&gt;<br /> | <br /> Soluble fraction of cell lysate<br /> <br /> Ni Magnetic beads<br /> <br /> | <br /> 9.76 / 52.8<br /> <br /> <br /> <br /> |}<br /> <br /> <br /> <br /> Ni megatic beads are not good. 2031&amp;nbsp;pCold-Tat-yFLP has better expression than 2032 pCold-Tat-Flpo<br /> <br /> <br /> [[Category:Protein Purification]]</div>

Paul.xu