https://eln.excellgen.com/index.php?action=history&feed=atom&title=2035-pLN6H-Tth-RNase-H 2035-pLN6H-Tth-RNase-H - Revision history 2026-10-18T08:50:07Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2035-pLN6H-Tth-RNase-H&diff=11245&oldid=prev Paul.xu: Created page with "== '''Cloning of 2035-pLN6H-Tth-RNase-H''' == '''Purpose:''' Cloning of 2035-pLN6H-Tth-RNase-H <br> '''Date: '''2014-2-12---2014-2-14 '''Notebook:0''' '''Page:''' '''Clon..." 2014-02-14T21:46:08Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2035-pLN6H-Tth-RNase-H&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2035-pLN6H-Tth-RNase-H &lt;br&gt; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-12---2014-2-14 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clon...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2035-pLN6H-Tth-RNase-H&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2035-pLN6H-Tth-RNase-H &lt;br&gt;<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-12---2014-2-14<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2035&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2016_2029_2030_2033_2034_2035pcr_1-23-2014.JPG]]&lt;br&gt;<br /> <br /> PCR from Thermus thermophilus HB8 genomic DNA (-80 oC ATCC box)&lt;br&gt;Tth-RNaseH-Nde1-F&lt;br&gt;Tth-RNaseH-Xho1-R&lt;br&gt;<br /> <br /> [[Image:2033_2034_2035_2037clone_2-12-2014.JPG]]<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 2029 pLNC6H&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-14<br /> <br /> Make miniprep and digest with Nde1 + Xho1<br /> <br /> [[Image:2035_2037digest_2-14-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu