https://eln.excellgen.com/index.php?action=history&feed=atom&title=2039-pT7A3-6H-sso7d-wt-Taq-N 2039-pT7A3-6H-sso7d-wt-Taq-N - Revision history 2026-10-18T23:08:02Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2039-pT7A3-6H-sso7d-wt-Taq-N&diff=11113&oldid=prev Paul.xu: Created page with "== '''Cloning of 2039-pT7A3-6H-sso7d-wt-Taq-N''' == '''Purpose:''' Cloning of 2039-pT7A3-6H-sso7d-wt-Taq-N '''Date: '''2013-1-23------2013-1-27 '''Notebook:0''' '''Page:''..." 2014-01-28T16:13:25Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2039-pT7A3-6H-sso7d-wt-Taq-N&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2039-pT7A3-6H-sso7d-wt-Taq-N &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-23------2013-1-27 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2039-pT7A3-6H-sso7d-wt-Taq-N&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2039-pT7A3-6H-sso7d-wt-Taq-N<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-23------2013-1-27 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2039&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2037_2039pcr_1-23-2014.JPG]]&lt;br&gt;<br /> <br /> PCR N terminus Taq from 462 pTrc-Wt-Taq (from Xu Yan)&lt;br&gt;wt-Taq-Nde1-F&lt;br&gt;wt-Taq-HindIII-R&lt;br&gt;<br /> <br /> [[Image:2039clone_1-24-2014.JPG]]<br /> <br /> {| width=&quot;331&quot; height=&quot;116&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane &lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1846 pT7A3-6H-sso7d&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-27<br /> <br /> Make miniprep and digest with Nde1 + HindIII&lt;br&gt;<br /> <br /> [[Image:1915_1940_2039digest_1-27-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; [[Category:Cloning]]</div> Paul.xu