https://eln.excellgen.com/index.php?action=history&feed=atom&title=2042_pT7A3E-HC-sfGFP 2042 pT7A3E-HC-sfGFP - Revision history 2026-10-18T14:12:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2042_pT7A3E-HC-sfGFP&diff=11055&oldid=prev Paul.xu at 16:02, 24 January 2014 2014-01-24T16:02:14Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2042 pT7A3E-HC-sfGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2042 pT7A3E-HC-sfGFP&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2042&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> subclone sfGFP from 1565 to&amp;nbsp; 1279 pT7A3 ,using Nhe1 + HindIII <br /> <br /> [[Image:DIGEST FOR 2042 2043.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> Make miniprep, digest with Nhe1 + HindIII &lt;br&gt; <br /> <br /> [[Image:1326 1535 2018 2042 2043 2044.JPG]]&lt;br&gt; <br /> <br /> DIGEST WITH XbaI+NheI, should get a small band=&amp;gt;100bp&lt;br&gt;<br /> <br /> [[Image:1326 1535 2018 2042 2043 2044digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu