https://eln.excellgen.com/index.php?action=history&feed=atom&title=2044_pBad24-Tat-yFLP 2044 pBad24-Tat-yFLP - Revision history 2026-10-18T22:24:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2044_pBad24-Tat-yFLP&diff=11087&oldid=prev Paul.xu at 14:44, 27 January 2014 2014-01-27T14:44:59Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2044 pBad24-Tat-yFLP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2044 pBad24-Tat-yFLP&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2044&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> subclone Tat-yFLP from 2031 to&amp;nbsp; 545 pBad24 , using Nco1+ Sal1 to digest vector, using Nco1+ Xho1 to digest 2031&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2044 1326 1535 2018.JPG]][[Image:DIGEST FOR 1326 2018 2044.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> Make miniprep, digest with Hindiii&lt;br&gt; <br /> <br /> [[Image:2044digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu