https://eln.excellgen.com/index.php?action=history&feed=atom&title=2045-pUC191 2045-pUC191 - Revision history 2026-10-18T16:05:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2045-pUC191&diff=11182&oldid=prev Paul.xu: Created page with "== '''Cloning of 2045-pUC191''' == '''Purpose:''' Cloning of 2045-pUC191 '''Date: '''2014-1-30--2014-2-3 '''Notebook:0''' '''Page:''' '''Clone ID:2045''' '''Experimen..." 2014-02-07T19:37:42Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2045-pUC191&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2045-pUC191 &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-30--2014-2-3 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2045&#039;&#039;&#039; &#039;&#039;&#039;Experimen...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2045-pUC191&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2045-pUC191 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-30--2014-2-3 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2045&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:726 727 2046 2060 718 2045pcr 1-31-2014.JPG]]&lt;br&gt; <br /> <br /> gene synthesis&lt;br&gt;<br /> <br /> pEC2-1 to pEC2-4&lt;br&gt;<br /> <br /> [[Image:718 2012 2014 2040 2045 2060clone 1-31-2014.JPG]] <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 9&lt;br&gt; <br /> | 10&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pcr &lt;br&gt; <br /> | puc19&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Sap1 + HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &#039;&#039;&#039;pcr product smeared after digestion&#039;&#039;&#039;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 2045-pUC191&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2045-pUC191<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-5---2014-2-7 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2045&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2029_2039_2045_2047_2067_2069pcr_2-5-2014.JPG]]&lt;br&gt; <br /> <br /> gene synthesis<br /> <br /> pEC2-1 to pEC2-4&lt;br&gt;<br /> <br /> [[Image:2045clone_2-7-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr,smeared&lt;br&gt;<br /> | puc19&lt;br&gt;<br /> |-<br /> | colspan=&quot;3&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Sap1 + HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 201<br /> <br /> Make miniprep and digest with&amp;nbsp; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu