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2026-10-18T16:06:51Z
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Paul.xu at 16:26, 20 November 2017
2017-11-20T16:26:23Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 2046-pET28b-Tat-NLS-yFlp-6H''' ==<br />
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'''Purpose:''' Cloning of 2046-pET28b-Tat-NLS-yFlp-6H<br />
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'''Date: '''2014-1-30---2014-2-3<br />
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'''Notebook:0'''<br />
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'''Page:'''<br />
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'''Clone ID:2046'''<br />
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'''Experimenters:''' yingying<br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[File:726 727 2046 2060 718 2045pcr 1-31-2014.JPG|RTENOTITLE]]<br />
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fragment 1:<br />
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template:pET28b-yFLP<br/>yFlp-Nde1-F<br/>yFlp-R1<br/><br />
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<br/>fragment 2:<br />
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template:pET28b-yFLP<br/>yFlp-F1<br/>yFlp-6His-Xho1-R<br />
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[[File:726 727 2046PCR 1-31-2014.JPG|RTENOTITLE]]<br />
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pcr1+pcr2<br />
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[[File:726 727 2046clone 1-31-2014.JPG|RTENOTITLE]]<br />
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{| width="200" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| lane<br/><br />
| 5<br/><br />
| 6<br/><br />
|-<br />
| <br/><br />
| pcr<br/><br />
| pET28b<br/><br />
|-<br />
| <br/><br />
| colspan="2" | Nde1 + Xho1<br/><br />
|}<br />
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=== Colony Screening ===<br />
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Date: 2014-2-3<br />
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Make miniprep and digest with Nde1 + Xho1<br />
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[[File:2046 2060digest 2-3-2014.JPG|RTENOTITLE]]<br />
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<br />
[[File:2046 digestion1.JPG]]<br />
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[[File:2046 digestion2.JPG]]<br />
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--[[User:Paul.xu|Paul.xu]] ([[User talk:Paul.xu|talk]]) 11:26, 20 November 2017 (EST)<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu