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2026-10-18T12:27:11Z
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Paul.xu at 13:30, 12 March 2014
2014-03-12T13:30:41Z
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<p><b>New page</b></p><div>== '''Cloning of 2048-pEB-C6H''' ==<br />
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'''Purpose:''' Cloning of 2048-pEB-C6H <br />
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'''Date: '''2014-3-5---2014-3-7 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:2048''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:2048pcr 2-27-2014.JPG]]<br> <br />
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pcr1:pBadE-1 to pBadE-4<br> <br />
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pcr2:PCR fragment from the vector using primers<br>pBad-Age1-F<br>pBad-R1<br> <br />
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[[Image:2048 2093PCR 2-28-2014.JPG]]<br> <br />
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PCR:pBadE-4 +pBad-Age1-F<br> <br />
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[[Image:2014 2048 2078clone 3-5-2014.JPG]]<br> <br />
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{| width="200" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| lane<br> <br />
| 3<br> <br />
| 4<br><br />
|-<br />
| <br> <br />
| pcr<br> <br />
| 545 pBad24<br><br />
|-<br />
| <br> <br />
| colspan="2" | Agei + HindIII<br><br />
|}<br />
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=== Colony Screening ===<br />
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Date: 2014-3-7 <br />
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Make miniprep and digest with&nbsp;Xho1 + EcoRV <br> <br />
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[[Image:2048digest 3-10-2014.JPG]]<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu