https://eln.excellgen.com/index.php?action=history&feed=atom&title=205_pET28-MBP-TurboTEV 2026-10-18T11:23:31Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=205_pET28-MBP-TurboTEV&diff=4031&oldid=prev 2012-04-30T19:09:06Z

<p></p> <p><b>New page</b></p><div>Cloning of pET28-MBP-TurboTEV&lt;br&gt; <br /> <br /> By Paul&lt;br&gt; <br /> <br /> 4/17/2012: &lt;br&gt; <br /> <br /> PCR MBP from pDB.His.MBP&lt;br&gt;MBP-NdeI-F AGT CAC ATA TGA AAA CTG AAG AAG GTA AAC TG&lt;br&gt;MBP-EcoR1-R TCG ATG AAT TCA GTC TGC GCG TCT TTC AGG G&lt;br&gt; <br /> <br /> [[Image:1063 digestion 4 17 2012.jpg]]&lt;br&gt; <br /> <br /> 4/17/2012&lt;br&gt; <br /> <br /> digest vector 203 with Nde1+ EcoR1 o/n&lt;br&gt; <br /> <br /> digest PCR product with Nde1 + EcoR1 o/n&lt;br&gt; <br /> <br /> 4/18/2012&lt;br&gt; <br /> <br /> [[Image:205 digestion 4 18 2012.jpg]]&lt;br&gt; <br /> <br /> 1: vector 203, pET28-TurboTEV&lt;br&gt; <br /> <br /> 2, 3: PCR product <br /> <br /> gel purify, ligate and transform NEB DH5plah&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> digest with Nde1+ EcoR1, clone into pET28-TurboTev 203&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Select 6 clone to do PCR screening&lt;br&gt; <br /> <br /> [[Image:1063 205 PCR Screening 4.19.2012.JPG]] <br /> <br /> Select 205-1,-2,-3 clones to mini-prep, digest <br /> <br /> [[Image:1063 205 digest 4.20.2012.JPG]] <br /> <br /> 205 PCR Screening 4/20/2012 <br /> <br /> [[Image:205 PCR Screening 4.20.2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> 4/26/2012&lt;br&gt; <br /> <br /> [[Image:203i 1116v digest 4.26.2012.JPG]]&lt;br&gt; <br /> <br /> Select 4 clones to mini-prep&lt;br&gt;205 plasmids are digested by EcoR1 + Sal1&lt;br&gt; <br /> <br /> [[Image:205 digest 4.30.2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

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