https://eln.excellgen.com/index.php?action=history&feed=atom&title=2068_pT7A3-6H-ChiBD-N-NpuDnaE-C1A 2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A - Revision history 2026-10-18T20:17:47Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2068_pT7A3-6H-ChiBD-N-NpuDnaE-C1A&diff=11403&oldid=prev Paul.xu: Created page with "== '''Cloning of 2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A''' == '''Purpose:''' Cloning of2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A'''==''' '''Date: '''2014-2---- '''Notebook:0''' '''Pag..." 2014-03-06T22:04:19Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Pag...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2068&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> <br /> <br /> PCR NpuDnaE-N from 1742 p8H-ELK-IN&lt;br&gt;GS-NpuDnaE-N-Nde1-F&lt;br&gt;atcatc cat atg ggaggaggtgggtctggaggtggaggatct GCg TTAAGCTATGAAACGGAAATATTG&lt;br&gt; Xho1&lt;br&gt;NpuDnaE-N-12 TGTACGCTCGAGTTAATTCGGCAAATTATCAACCCGCATCAAATCC<br /> <br /> &lt;br&gt;[[Image:2028_2062firstpcr_2068pcr.JPG]]&lt;br&gt; <br /> <br /> digest with Nde1 + XhoI clone to 2067 pT7A3-6H-CBD&lt;br&gt; <br /> <br /> [[Image:Pcrdigest for 2064 2065 2068.JPG]][[Image:2067digest_for_2068.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014 <br /> <br /> Make miniprep, digest with Nde1 + XhoI <br /> <br /> [[Image:1980_2066_2068digest.JPG]]<br /> <br /> [[Image:2050_2068_2083_2100digest&#039;.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu