https://eln.excellgen.com/index.php?action=history&feed=atom&title=2069-pEC-ha-Plac-hia5 2069-pEC-ha-Plac-hia5 - Revision history 2026-10-18T08:50:31Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2069-pEC-ha-Plac-hia5&diff=11178&oldid=prev Paul.xu: Created page with "== '''Cloning of 2069-pEC-ha-Plac-hia5''' == '''Purpose:''' Cloning of 2069-pEC-ha-Plac-hia5 '''Date: '''2014--2-4---2014-2-7 '''Notebook:0''' '''Page:''' '''Clone ID:206..." 2014-02-07T19:19:50Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2069-pEC-ha-Plac-hia5&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2069-pEC-ha-Plac-hia5 &#039;&#039;&#039;Date: &#039;&#039;&#039;2014--2-4---2014-2-7 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:206...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2069-pEC-ha-Plac-hia5&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2069-pEC-ha-Plac-hia5<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014--2-4---2014-2-7<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2069&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2029_2039_2045_2047_2067_2069pcr_2-5-2014.JPG]]<br /> <br /> PCR using these two oligos using 1838 pEC-hia5&lt;br&gt;ha-constitutive-PLac-F A3 &lt;br&gt;ha-constitutive-PLac-Hia5-F&lt;br&gt;<br /> <br /> [[Image:2069_2072_2073clone_2-5-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1454 pEC&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | HindIII + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-7 <br /> <br /> Make miniprep and digest with HindIII + Xho1<br /> <br /> [[Image:2069digest_2-7-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu