https://eln.excellgen.com/index.php?action=history&feed=atom&title=2076-pCMV5-RSV-Gag-NLS-Flp-WPRE-oriP 2026-10-18T08:22:43Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2076-pCMV5-RSV-Gag-NLS-Flp-WPRE-oriP&diff=11419&oldid=prev 2014-03-07T21:14:54Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2076-pCMV5-RSV-Gag-NLS-Flp-WPRE-oriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2076-pCMV5-RSV-Gag-NLS-Flp-WPRE-oriP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;20142-23---2014-2-27 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2076&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2015 2076pcr 2-21-2014.JPG]] <br /> <br /> [[Image:2012 2014 2015 2076 2078PCR 2-24-2014.JPG]] <br /> <br /> [[Image:2012 2015 2076 2082clone 2-25-2014.JPG]] <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane &lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | BamH1+ Kpn1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-27 <br /> <br /> Make miniprep and digest with BamH1+ Kpn1<br /> <br /> [[Image:2015 2076digest 2-27-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu