https://eln.excellgen.com/index.php?action=history&feed=atom&title=2078-pEC2-DNA-Ladder 2078-pEC2-DNA-Ladder - Revision history 2026-10-18T07:50:15Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2078-pEC2-DNA-Ladder&diff=11514&oldid=prev Paul.xu at 18:06, 21 March 2014 2014-03-21T18:06:38Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2078-pEC2-DNA-Ladder&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2078-pEC2-DNA-Ladder <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-3-2---2014-3-7 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2078&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2078pcr 2-19-2014.JPG]]&lt;br&gt; <br /> <br /> pcr1 using lambda DNA as template <br /> <br /> Fragment 1: 370 bp&lt;br&gt;1 ul of 10 uM mXho1-BamH1-R &lt;br&gt;1 ul of 1 uM DNA-ladder-F &lt;br&gt;1 ul of 10 uM DNA-ladder-R1&lt;br&gt; <br /> <br /> &amp;nbsp;&lt;br&gt; <br /> <br /> pcr2 using lambda DNA as template&lt;br&gt; <br /> <br /> Fragment 2: 799 bp&lt;br&gt;DNA-ladder-F1&lt;br&gt;DNA-ladder-R2&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> pcr3 using lambda DNA as template&lt;br&gt; <br /> <br /> Fragment 3: 737 bp&lt;br&gt;DNA-ladder-F2 &lt;br&gt;CTCATCACCCCCAAGTCTGGC AAGCTT TGAATGCCTGGAATTAATCAC&lt;br&gt;DNA-ladder-Xho1-R&lt;br&gt; <br /> <br /> [[Image:2012 2014 2015 2076 2078PCR 2-24-2014.JPG]]&lt;br&gt; <br /> <br /> PCR:pcr1+pcr2_pcr3&lt;br&gt; <br /> <br /> [[Image:2014 2048 2078clone 3-5-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;271&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR&lt;br&gt; <br /> | 2015 pEC2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | BamH1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === colony Screening ===<br /> <br /> Date: 2013-1-24 <br /> <br /> Make miniprep and digest with Hind111&lt;br&gt; <br /> <br /> [[Image:2040clone+2078digest 3-7-2014.JPG]][[Image:Lambda EcoR1 HindIII.jpg]]&lt;br&gt; <br /> <br /> [[Image:2078digest_3-21-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;311&quot; height=&quot;107&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> | 3&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;3&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; 2078-1&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | BamH1&lt;br&gt;<br /> | Hind111&lt;br&gt;<br /> | <br /> BamH1/Hind111<br /> <br /> mix 1 and 2<br /> <br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu