https://eln.excellgen.com/index.php?action=history&feed=atom&title=2079-pLVX-p53 2079-pLVX-p53 - Revision history 2026-10-18T23:24:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2079-pLVX-p53&diff=11305&oldid=prev Paul.xu: Created page with "== '''Cloning of 2079-pLVX-p53''' == '''Purpose:''' Cloning of 2079-pLVX-p53 '''Date: '''2014-2-19---2014-2-21 '''Notebook:0''' '''Page:''' '''Clone ID:2079''' '''Exper..." 2014-02-21T19:08:20Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2079-pLVX-p53&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2079-pLVX-p53 &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-19---2014-2-21 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2079&#039;&#039;&#039; &#039;&#039;&#039;Exper...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2079-pLVX-p53&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2079-pLVX-p53<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-19---2014-2-21<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2079&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2079PCR_2-19-2014.JPG]]PCR from 80 pLex-p53wt&lt;br&gt;PCR from 1341-pDB.His.MBP-(3C)-p53wt&lt;br&gt;p53-EcoR1-Nde1-F &lt;br&gt;P53-Xho1-BamH1-R1<br /> <br /> [[Image:2074_2079clone_2-19-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 153 pLVX-Plus&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | EcoR1 + BamH1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-21<br /> <br /> Make miniprep and digest with EcoR1 + BamH1&lt;br&gt;<br /> <br /> [[Image:2079digest2-21-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu