https://eln.excellgen.com/index.php?action=history&feed=atom&title=2083_pFastbac1-p53 2083 pFastbac1-p53 - Revision history 2026-10-18T22:23:22Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2083_pFastbac1-p53&diff=11380&oldid=prev Paul.xu at 21:20, 6 March 2014 2014-03-06T21:20:34Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2083 pFastbac1-p53&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2083 pFastbac1-p53&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2083&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from1341&lt;br&gt; <br /> <br /> p53-EcoR1-Nde1-F atcgac gaattc gcc acc cat ATGGAGGAGCCGCAGTCAGATC <br /> <br /> p53-Xho1-6H-HindIII-R&lt;br&gt;(GACAGAAGGGCCTGACTCAGAC ctcgag CAT CAT CAT CAT CAT CAC TGA AAGCTT tactac)&lt;br&gt;gtagtaAAGCTTTCAGTGATGATGATGATGATGctcgagGTCTGAGTCAGGCCCTTCTGTC <br /> <br /> [[Image:2083 2100pcr.JPG]]&lt;br&gt; <br /> <br /> digest with EcoR1 + HindIII <br /> <br /> [[Image:Digest for2106 2083 2100.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014 <br /> <br /> Make miniprep, digest with EcoR1 + HindIII <br /> <br /> [[Image:2050 2068 2083 2100digest&#039;.JPG]] <br /> <br /> sent 2083-2 to sequence,&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu