https://eln.excellgen.com/index.php?action=history&feed=atom&title=2087-pT7A3-MBP-sso7d-6H 2087-pT7A3-MBP-sso7d-6H - Revision history 2026-10-18T21:36:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2087-pT7A3-MBP-sso7d-6H&diff=11349&oldid=prev Paul.xu: Created page with "== '''Cloning of 2087-pT7A3-MBP-sso7d-6H''' == '''Purpose:''' Cloning of 2087-pT7A3-MBP-sso7d-6H '''Date: '''2014-2-24---2014-2-27 '''Notebook:0''' '''Page:''' '''Clone ..." 2014-03-04T18:44:51Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2087-pT7A3-MBP-sso7d-6H&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2087-pT7A3-MBP-sso7d-6H &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-24---2014-2-27 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2087-pT7A3-MBP-sso7d-6H&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2087-pT7A3-MBP-sso7d-6H <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-24---2014-2-27<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2087&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2077_2079_2082_2087_2088_2092PCR_2-21-2014.JPG]]<br /> <br /> PCR MBP from 1865 pT7A3-MBP-FluA-NP&lt;br&gt;using &lt;br&gt;MBP-NdeI-F&lt;br&gt;MBP-Xho1-R&lt;br&gt;<br /> <br /> [[Image:2087_2088_2092_2107clone_2-25-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;396&quot; height=&quot;127&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | <br /> &lt;br&gt;<br /> <br /> 1847 pT7A30-sso7d-6H&lt;br&gt;<br /> <br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-27<br /> <br /> Make miniprep and digest with&amp;amp;nbsp; Nde1 + Xho1 <br /> <br /> [[Image:2087_2092digest_2-27-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu