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Paul.xu at 18:46, 4 March 2014
2014-03-04T18:46:06Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 2092-pT7A3-MBP-i3C-6H''' ==<br />
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'''Purpose:''' Cloning of 2092-pT7A3-MBP-i3C-6H <br />
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'''Date: '''2014-2-25----2014-2-27 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:2092''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:2077_2079_2082_2087_2088_2092PCR_2-21-2014.JPG]]<br><br />
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PCR MBP from 1804 pCold-MBP-(E3C)<br />
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3_11_2013<br>Pos B08<br>SequenceName MBP-Nco1-F<br>MBP-(EK-TEV)-Nde1-R<br><br />
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[[Image:2087 2088 2092 2107clone 2-25-2014.JPG]] <br />
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{| width="277" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| lane<br> <br />
| 5<br> <br />
| 6<br><br />
|-<br />
| <br> <br />
| pcr<br> <br />
| 1601 pT7A3-6His-GST-i3C<br><br />
|-<br />
| <br> <br />
| colspan="2" | Nde1 + Nco1<br><br />
|}<br />
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=== Colony Screening ===<br />
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Date: 2014-2-27 <br />
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Make miniprep and digest with Nde1 + Xho1 <br />
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[[Image:2087_2092digest_2-27-2014.JPG]]<br> <br />
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&lt;br&gt; <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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<br> <br />
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[[Category:Cloning]]</div>
Paul.xu