https://eln.excellgen.com/index.php?action=history&feed=atom&title=2101-pT7A3-sso7d 2101-pT7A3-sso7d - Revision history 2026-10-18T16:58:54Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2101-pT7A3-sso7d&diff=11771&oldid=prev Paul.xu: Created page with "== '''Cloning of 2101-pT7A3-sso7d''' == '''Purpose:''' Cloning of 2101-pT7A3-sso7d '''Date: '''2014-4--26---2014-4-28 '''Notebook:0''' '''Page:''' '''Clone ID:2101''' '..." 2014-04-30T18:36:08Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2101-pT7A3-sso7d&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2101-pT7A3-sso7d &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4--26---2014-4-28 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2101&#039;&#039;&#039; &#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2101-pT7A3-sso7d&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2101-pT7A3-sso7d<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4--26---2014-4-28<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2101&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2033_2093_2101_2169_2185PCR_4-25-2014.JPG]]<br /> <br /> [[Image:2033_2085_2093_2101_2163clone_4-25-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | 7&lt;br&gt;<br /> | 8&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1279,pT7A3&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | Nco1 + Sal1&lt;br&gt;<br /> | Nco1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-28<br /> <br /> Make miniprep and digest with EcoRV + Ecor1, linearized vector (not two bands) <br /> <br /> [[Image:2101_2169digest_4-28-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu