Paul.xu: Undo revision 16630 by Paul.xu (talk)

2017-11-19T18:37:39Z

Undo revision 16630 by Paul.xu (talk)

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PageRuler Plus 10, 15, 25(red), 35, 55, 70(red), 100, 130, 250

[[File:2106 2115 CRE.jpg|RTENOTITLE]]

{| width="200" border="1" cellspacing="1" cellpadding="1"
|-
| lane 
| colspan="5" | 1 
| colspan="2" | 2 
| 3 
| 4 
| 5 
|-
| clone ID
| colspan="5" |
2106

pET28b-iFLP

| colspan="2" |
2115

pT7A3-iFLP

| PageRuler Plus Prestained protein ladder 
| colspan="2" | 1708+1823 
|-
| host 
| colspan="5" | Nico21DE3plysN 
| colspan="2" | Nico21DE3plysN 
| 
| colspan="2" | Nico21DE3plysN 
|-
| induction 
| colspan="5" | 0.2 mM IPTG 25 oC o/n 5ml 
| colspan="2" | 0.2 mM IPTG 25 oC o/n  5ml 
| 
| colspan="2" | 
|-
| purification 
| colspan="5" | Ni beads 
| colspan="2" | Ni beads 
| 
| colspan="2" | Ni Column-- SpFF--SpFF 
|-
| MW 
| colspan="5" | pI/Mw: 9.76 / 52.8 
| colspan="2" | pI/Mw: 9.76 / 52.8 
| 
| colspan="2" | 10.30 / 42.8 
|}
[[Category:Protein Purification]]

2106 2115 cre - Revision history

Paul.xu: Undo revision 16630 by Paul.xu (talk)