https://eln.excellgen.com/index.php?action=history&feed=atom&title=2108-pCold-Tat-NLS-iFLP-6H 2108-pCold-Tat-NLS-iFLP-6H - Revision history 2026-10-19T02:32:17Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2108-pCold-Tat-NLS-iFLP-6H&diff=11501&oldid=prev Paul.xu: Created page with "== '''Cloning of 2108-pCold-Tat-NLS-iFLP-6H''' == '''Purpose:''' Cloning of 2108-pCold-Tat-NLS-iFLP-6H '''Date: '''2014-3-14---2014-3-17 '''Notebook:0''' '''Page:''' '''..." 2014-03-19T18:33:11Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2108-pCold-Tat-NLS-iFLP-6H&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2108-pCold-Tat-NLS-iFLP-6H &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-3-14---2014-3-17 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2108-pCold-Tat-NLS-iFLP-6H&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2108-pCold-Tat-NLS-iFLP-6H<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-3-14---2014-3-17 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2108&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2108PCR_3-14-2014.JPG]]<br /> <br /> PCR using &lt;br&gt;yFlp-Nde1-F&lt;br&gt;iFlp-6His-Xho1-R &lt;br&gt;template is 2106 pET28b-iFLP&lt;br&gt;<br /> <br /> [[Image:2108clone_3-14-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 2047 pCold-C6H&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + Xho1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-3-17<br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:2108digest_3-17-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu