https://eln.excellgen.com/index.php?action=history&feed=atom&title=2110_pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe 2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe - Revision history 2026-10-18T17:27:48Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2110_pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe&diff=11959&oldid=prev Paul.xu: Created page with "== '''Cloning of 2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe''' == '''Purpose:''' Cloning of 2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe '''Date: '''2014-6 '''Notebook:0'..." 2014-06-10T15:38:39Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6 &#039;&#039;&#039;Notebook:0&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2110&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning&lt;br&gt; ===<br /> <br /> subclone CMV-Flpe from 471 pAAV.CMV-FLPe using Not1 + Xba1 to 2226 <br /> <br /> 471digest with Not1 + Xba1+BsaI,CUT the first band <br /> <br /> [[Image:Digest for 2110 1403.JPG]] <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-6 <br /> <br /> mini prep and digest with EcoR1 <br /> <br /> [[Image:2110DIGEST.JPG]]<br /> <br /> &lt;br&gt; verify using Not1 + Xba1 <br /> <br /> [[Image:1403 2110digest.JPG]] <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu