https://eln.excellgen.com/index.php?action=history&feed=atom&title=2112_pFastBac-Dual-VSVG-HIVGag-iCre-CMV-iCre 2112 pFastBac-Dual-VSVG-HIVGag-iCre-CMV-iCre - Revision history 2026-10-18T15:07:59Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2112_pFastBac-Dual-VSVG-HIVGag-iCre-CMV-iCre&diff=11991&oldid=prev Paul.xu at 19:18, 12 June 2014 2014-06-12T19:18:47Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2112 pFastBac-Dual-VSVG-HIVGag-iCre-CMV-iCre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2112 pFastBac-Dual-VSVG-HIVGag-iCre-CMV-iCre <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2112&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning&lt;br&gt; ===<br /> <br /> subclone CMV-NLS-Cre from 2239 pAAV-CMV-iCre using Not1 + HindIII to 2099pFastBac-Dual-VSVG-HIV-Gag-Cre <br /> <br /> 2239digest with Not1 + Xba1+BsaI,CUT the first band <br /> <br /> [[Image:Digest for 2112 22243nd.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-6 <br /> <br /> mini prep and digest with EcoR1 <br /> <br /> [[Image:2112 2227 2228digest.JPG]] <br /> <br /> &lt;br&gt; verify using Not1 + Xba1 and BamHI&lt;br&gt; <br /> <br /> [[Image:1588REAL 2112 2227 2228DIGEST.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu