https://eln.excellgen.com/index.php?action=history&feed=atom&title=2115_2108_1142_1992 2115 2108 1142 1992 - Revision history 2026-10-18T11:51:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2115_2108_1142_1992&diff=12047&oldid=prev Paul.xu at 21:25, 19 June 2014 2014-06-19T21:25:55Z <p></p> <p><b>New page</b></p><div>06/17/2014 <br /> <br /> [[Image:2115 2108 1142 1992.jpg]]&lt;br&gt; <br /> <br /> {| width=&quot;832&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | sample&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;7&quot; | 2115 <br /> pT7A3-iFLP&lt;br&gt; <br /> <br /> pI/Mw: 9.76 / 52.8 &lt;br&gt; &lt;br&gt; <br /> <br /> | rowspan=&quot;7&quot; | iFLP &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;7&quot; | 2115 &lt;br&gt; &lt;br&gt; <br /> | <br /> 15 ml,&lt;br&gt; <br /> <br /> Tuner(DE3)-pG-KJE8 <br /> <br /> | <br /> &lt;br&gt; <br /> <br /> 50uM IPTG&amp;nbsp; 20oC o/n <br /> <br /> | <br /> Nickel Magnetic Bead <br /> <br /> | <br /> S&lt;br&gt; <br /> <br /> |-<br /> | 2&lt;br&gt; <br /> | <br /> 1 5 ml, <br /> <br /> Tuner(DE3)-pGro7 &lt;br&gt; <br /> <br /> | <br /> 50uM IPTG 20oC o/n <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> Nickel Magnetic Bead <br /> <br /> | <br /> S&lt;br&gt; <br /> <br /> |-<br /> | 3&lt;br&gt; <br /> | <br /> 15 ml, <br /> <br /> Tuner(DE3)-pKJE7 &lt;br&gt; <br /> <br /> | <br /> 50uM IPTG 20oC o/n <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> Nickel Magnetic Bead <br /> <br /> | <br /> S <br /> <br /> |-<br /> | 4&lt;br&gt; <br /> | <br /> 15 ml, <br /> <br /> Tuner(DE3)-pGTf2&lt;br&gt; <br /> <br /> | <br /> 50uM IPTG 20oC o/n <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> Nickel Magnetic Bead <br /> <br /> | <br /> S <br /> <br /> |-<br /> | 5&lt;br&gt; <br /> | 15 ml, <br /> Tuner(DE3)-pTf16&lt;br&gt; <br /> <br /> | <br /> 50uM IPTG 20oC o/n <br /> <br /> &lt;br&gt; <br /> <br /> | Nickel Magnetic Bead&lt;br&gt; <br /> | S&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | Nico21DE3plysN &lt;br&gt; <br /> | 50uM IPTG 20oC o/n&lt;br&gt; <br /> | <br /> Nickel Magnetic Bead&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> &lt;br&gt; <br /> <br /> |-<br /> | 7&lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> TunerDE3plysN &lt;br&gt; <br /> <br /> | 50uM IPTG 20oC o/n&lt;br&gt; <br /> | <br /> Nickel Magnetic Bead <br /> <br /> | <br /> s <br /> <br /> |-<br /> | 8&lt;br&gt; <br /> | pCold-Tat-NLS-iFLP-6H pT7A3-iFLP <br /> 9.76 / 52.8 &lt;br&gt; <br /> <br /> | iFLP&lt;br&gt; <br /> | 2108&lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> TunerDE3plysN &lt;br&gt; <br /> <br /> | 50uM IPTG 20oC o/n&lt;br&gt; <br /> | Nickel Magnetic Bead&lt;br&gt; <br /> | <br /> s&lt;br&gt; <br /> <br /> |-<br /> | &lt;br&gt; <br /> | PageRuler Plus Prestained protein ladder&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> pLN6H-Tth-SSB&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> <br /> 5.7/31.1 <br /> <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1142&lt;br&gt; <br /> | Nico21DE3plysN&lt;br&gt; <br /> | grow at 30oC, induce at 42oC o/n&lt;br&gt; <br /> | Nickel Magnetic Bead &lt;br&gt; <br /> | s&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> pL42-SM2 &lt;br&gt; <br /> <br /> 6.54/27.9&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1992&lt;br&gt; <br /> | Nico21DE3plysN&lt;br&gt; <br /> | grow at 30oC, induce at 42oC o/n&lt;br&gt; <br /> | Nickel Magnetic Bead&lt;br&gt; <br /> | s&lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | MBP-Taq-Muts&lt;br&gt; <br /> | &lt;br&gt; <br /> | 211&lt;br&gt; <br /> | &lt;br&gt; <br /> | Eluted from NI column&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Image:2115 2108 1142 1992p.jpg]] <br /> <br /> {| width=&quot;832&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | sample&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | rowspan=&quot;7&quot; | 2115 <br /> pT7A3-iFLP&lt;br&gt; <br /> <br /> pI/Mw: 9.76 / 52.8 &lt;br&gt; &lt;br&gt; <br /> <br /> | rowspan=&quot;7&quot; | iFLP &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;7&quot; | 2115 &lt;br&gt; &lt;br&gt; <br /> | <br /> 15 ml,&lt;br&gt; <br /> <br /> Tuner(DE3)-pG-KJE8 <br /> <br /> | <br /> &lt;br&gt; <br /> <br /> 50uM IPTG&amp;nbsp; 20oC o/n <br /> <br /> | <br /> Nickel&amp;nbsp; Bead <br /> <br /> | <br /> p&lt;br&gt; <br /> <br /> |-<br /> | 3&lt;br&gt; <br /> | <br /> 1 5 ml, <br /> <br /> Tuner(DE3)-pGro7 &lt;br&gt; <br /> <br /> | <br /> 50uM IPTG 20oC o/n <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> Nickel&amp;nbsp; Bead <br /> <br /> | <br /> p&lt;br&gt; <br /> <br /> |-<br /> | 4&lt;br&gt; <br /> | <br /> 15 ml, <br /> <br /> Tuner(DE3)-pKJE7 &lt;br&gt; <br /> <br /> | <br /> 50uM IPTG 20oC o/n <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> Nickel Bead <br /> <br /> | <br /> p <br /> <br /> |-<br /> | 5&lt;br&gt; <br /> | <br /> 15 ml, <br /> <br /> Tuner(DE3)-pGTf2&lt;br&gt; <br /> <br /> | <br /> 50uM IPTG 20oC o/n <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> Nickel&amp;nbsp; Bead <br /> <br /> | <br /> p <br /> <br /> |-<br /> | 6&lt;br&gt; <br /> | 15 ml, <br /> Tuner(DE3)-pTf16&lt;br&gt; <br /> <br /> | <br /> 50uM IPTG 20oC o/n <br /> <br /> &lt;br&gt; <br /> <br /> | Nickel&amp;nbsp; Bead&lt;br&gt; <br /> | p&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | Nico21DE3plysN &lt;br&gt; <br /> | 50uM IPTG 20oC o/n&lt;br&gt; <br /> | <br /> Nickel Bead&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> &lt;br&gt; <br /> <br /> |-<br /> | 8&lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> TunerDE3plysN &lt;br&gt; <br /> <br /> | 50uM IPTG 20oC o/n&lt;br&gt; <br /> | <br /> Nickel&amp;nbsp; Bead <br /> <br /> | <br /> p <br /> <br /> |-<br /> | 9&lt;br&gt; <br /> | pCold-Tat-NLS-iFLP-6H pT7A3-iFLP <br /> 9.76 / 52.8 &lt;br&gt; <br /> <br /> | iFLP&lt;br&gt; <br /> | 2108&lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> TunerDE3plysN &lt;br&gt; <br /> <br /> | 50uM IPTG 20oC o/n&lt;br&gt; <br /> | Nickel&amp;nbsp; Bead&lt;br&gt; <br /> | <br /> p&lt;br&gt; <br /> <br /> |-<br /> | &lt;br&gt; <br /> | PageRuler Plus Prestained protein ladder&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> pLN6H-Tth-SSB&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> <br /> 5.7/31.1 <br /> <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1142&lt;br&gt; <br /> | Nico21DE3plysN&lt;br&gt; <br /> | grow at 30oC, induce at 42oC o/n&lt;br&gt; <br /> | Nickel&amp;nbsp; Bead &lt;br&gt; <br /> | p&lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> pL42-SM2 &lt;br&gt; <br /> <br /> 6.54/27.9&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1992&lt;br&gt; <br /> | Nico21DE3plysN&lt;br&gt; <br /> | grow at 30oC, induce at 42oC o/n&lt;br&gt; <br /> | Nickel Bead&lt;br&gt; <br /> | p&lt;br&gt;<br /> |-<br /> | 12&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu