https://eln.excellgen.com/index.php?action=history&feed=atom&title=212-pDBHisMBP-TthMuts 2026-10-18T12:51:45Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=212-pDBHisMBP-TthMuts&diff=4374&oldid=prev 2012-06-01T14:51:56Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 212-TthMuts&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of TthMuts by PCR <br /> <br /> &#039;&#039;&#039;Date:&amp;nbsp;&#039;&#039;&#039; &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 212&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenter:&#039;&#039;&#039; Jennifer Truong&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> Date: 5/24/2012&lt;br&gt; <br /> <br /> Notebook/page: <br /> <br /> PCR cloning using genomic DNA. <br /> <br /> [[Image:212 PCR 5 24 2012.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt;<br /> |-<br /> | Sample (Genomic DNA)&lt;br&gt; <br /> | HP8 &lt;br&gt; <br /> | HP27&lt;br&gt; <br /> | HP27&lt;br&gt;<br /> |-<br /> | Sample Lot&lt;br&gt; <br /> | &lt;br&gt; <br /> | 57761360&lt;br&gt; <br /> | 4728857&lt;br&gt;<br /> |}<br /> <br /> Cut out the ~2.4 kB band, add gel solubilization buffer. Purify and digest with Nde1 + EcoR1. Digest pDB.His.MBP with Nde1 + EcoR1.&lt;br&gt; <br /> <br /> 5/27/2012 <br /> <br /> digest miniprep with Nde1+ EcoR1. <br /> <br /> not good <br /> <br /> [[Image:212 1192 5 27 2012.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; PCR&amp;nbsp;Screening&lt;br&gt; <br /> <br /> Samples 1-8 <br /> <br /> [[Image:212 1-8 5 30 2012.JPG]] <br /> <br /> Possibly success in lane 3, but unclear. <br /> <br /> [[Image:212 9-16-2012530.jpg]]&lt;br&gt; <br /> <br /> There is no positive band for these samples (No 9 to 16 PCR samples) <br /> <br /> [[Image:212 9-26 2012530.JPG]]&lt;br&gt; <br /> <br /> No positive results&lt;br&gt; <br /> <br /> Experiment abandoned because there were no positive results from PCR&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> Experiment abandoned because there were no positive results from PCR screening. &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | Experiment abandoned because there were no positive results from PCR screening. &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

Paul.xu