https://eln.excellgen.com/index.php?action=history&feed=atom&title=2123-pCMV5-Mfge8-Tat-Cre-MFGE8 2123-pCMV5-Mfge8-Tat-Cre-MFGE8 - Revision history 2026-10-18T23:55:30Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2123-pCMV5-Mfge8-Tat-Cre-MFGE8&diff=11675&oldid=prev Paul.xu at 20:10, 11 April 2014 2014-04-11T20:10:25Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2123-pCMV5-Mfge8-Tat-Cre-MFGE8&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2123-pCMV5-Mfge8-Tat-Cre-MFGE8 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-10---2014-4-11 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2123&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2122 2123clone 4-10-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 1972 pCMV5-Tat-Cre-PDGFR&lt;br&gt; <br /> | 2128 pCMV5-Mfge8-Tat-TagGFP-MFGE8&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nhe1 + Kpn1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-11 <br /> <br /> Make miniprep and digest with Nhe1 + Kpn1 <br /> <br /> [[Image:2122_2123digest_4-11-2014.JPG]]&lt;br&gt; <br /> <br /> clone 5 right<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu