https://eln.excellgen.com/index.php?action=history&feed=atom&title=212_pL6H-TthMuts 2026-10-18T08:04:30Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=212_pL6H-TthMuts&diff=12969&oldid=prev 2014-08-26T17:17:14Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 212 pL6H-TthMuts&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of212 pL6H-TthMuts&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:212&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from HB8 genomic DNA using <br /> <br /> PCR using Tth Genomic DNA, digestion, ligation Tth-MutS-F ATC GCA TAT GCT CAA GGG CGA AGG CCC NdeI Tth-MutS-R (CTGGGTAGCATGAAGGGG TGA GAATTCATCG) CGA TGA ATT CTC ACC CCT TCA TGC TAC CCA G <br /> <br /> &lt;br&gt;[[Image:212 2198 22292ndpcr.JPG]]&lt;br&gt; <br /> <br /> digest with Nde1 + EcoR1, clone to1997&lt;br&gt; <br /> <br /> [[Image:Digest for 2300 2214 2198 212.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_ <br /> <br /> Make miniprep, use Nde1 + EcoR1&amp;nbsp; to find positive clone&lt;br&gt; <br /> <br /> [[Image:212_2198digest.JPG]]&lt;br&gt;<br /> <br /> digest with Nde1 + EcoR1+BamHI&lt;br&gt;<br /> <br /> [[Image:212_2198_2215-1_2276_2277_2278digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

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