https://eln.excellgen.com/index.php?action=history&feed=atom&title=2132_pEB-T4-Endo-IV 2132 pEB-T4-Endo-IV - Revision history 2026-10-18T19:26:12Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2132_pEB-T4-Endo-IV&diff=11552&oldid=prev Paul.xu: Created page with "= '''Cloning of 2132 pEB-T4-Endo-IV''' = = '''Purpose:''' Cloning of2132 pEB-T4-Endo-IV'''==''' '''Date: '''2014-3---- '''Notebook:0''' '''Page:''' '''Clone ID:2132''' ..." 2014-03-25T15:03:49Z <p>Created page with &quot;= &#039;&#039;&#039;Cloning of 2132 pEB-T4-Endo-IV&#039;&#039;&#039; = = &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2132 pEB-T4-Endo-IV&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-3---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2132&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>= &#039;&#039;&#039;Cloning of 2132 pEB-T4-Endo-IV&#039;&#039;&#039; = =<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2132 pEB-T4-Endo-IV&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-3---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2132&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> &amp;nbsp;1560-5 ,missing 1 G, can be fixed by PCR using primer&lt;br&gt;T4-Endo-IV-1 TAGCACCATATGCAGAAAACGAATCCTGGGTTACAGCGTCTGTTTCAGATTCCGACCT&lt;br&gt;T4-Endo-IV-14 GGTCGTACTCGAGAATGGAAAGATACCATCCGTTGTAGTTGCTTTTGC<br /> <br /> [[Image:2132 2062fix2.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> .digest with Nde + Xho1 clone to 2048 pEB-C6H&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_2_ <br /> <br /> Make miniprep, digestion with Nde + Xho1&lt;br&gt; <br /> <br /> [[Image:2062fix2 2132digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu