https://eln.excellgen.com/index.php?action=history&feed=atom&title=2133_pT7A3-ILK 2133 pT7A3-ILK - Revision history 2026-10-19T06:51:03Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2133_pT7A3-ILK&diff=11625&oldid=prev Paul.xu at 19:15, 5 April 2014 2014-04-05T19:15:57Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2133 pT7A3-ILK&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2133 pT7A3-ILK&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-3---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2133&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from Harvard Kinase HRG007900 32 H04 147913 HsCD00000155 FUSION pDNR-DUAL 3611 ILK 1359 0-1-0-0-1-0-1-0-0 &lt;br&gt;<br /> <br /> ILK-Nco1-Nhe1-F tagcac ccatg ggt agc agc gct agc GACGACATTTTCACTCAGTGC &lt;br&gt;<br /> <br /> ILK-Not1-Xho1-R (CTTGAGAAGATGCAGGACAAG CAC CAt CAC CAt CAC CAt TAG CGGCCG ctcgag atagca) tgctatctcgagCGGCCGCTAaTGGTGaTGGTGaTGGTGCTTGTCCTGCATCTTCTCAAG &lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2133pcr.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> cut with Nco1 + Xho, clone into 1279&lt;br&gt;<br /> <br /> [[Image:Digest_for_21332nd.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening&lt;br&gt; ===<br /> <br /> Date: 2014_3_ <br /> <br /> Make miniprep, digestion with Nco1 + Xho&lt;br&gt; <br /> <br /> ----<br /> <br /> [[Image:2133digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu