https://eln.excellgen.com/index.php?action=history&feed=atom&title=2134_pCMV5-ILK 2134 pCMV5-ILK - Revision history 2026-10-18T22:21:55Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2134_pCMV5-ILK&diff=11624&oldid=prev Paul.xu at 19:15, 5 April 2014 2014-04-05T19:15:51Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2134 pCMV5-ILK&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2134 pCMV5-ILK&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2134&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> <br /> subclone from 2133 pT7A3-ILK using Nhe1-Not1 to 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP <br /> <br /> [[Image:Digest for 2134 2136 2140.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening&lt;br&gt; ===<br /> <br /> Date: 2014_4_ <br /> <br /> Make miniprep, digestion with BamH1 + Kpn1&lt;br&gt; <br /> <br /> there are one BamHI and one KpnI site in ILK, so use BamH1 + Kpn1 to digest 2134,we will get four bands&lt;br&gt; <br /> <br /> [[Image:2134digest.JPG]]&lt;br&gt; <br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu