https://eln.excellgen.com/index.php?action=history&feed=atom&title=2142-pCMV5-p53-wt 2142-pCMV5-p53-wt - Revision history 2026-10-18T17:16:12Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2142-pCMV5-p53-wt&diff=11731&oldid=prev Paul.xu: Created page with "== '''Cloning of 2142-pCMV5-p53-wt''' == '''Purpose:''' Cloning of 1519-pT7A3-T3-DNA-Ligase '''Date: '''2014-4-21---2014-4-23 '''Notebook:0''' '''Page:''' '''Clone ID:2..." 2014-04-23T18:43:23Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2142-pCMV5-p53-wt&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1519-pT7A3-T3-DNA-Ligase &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-21---2014-4-23 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2142-pCMV5-p53-wt&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1519-pT7A3-T3-DNA-Ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-21---2014-4-23 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2142&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2141_2142PCR_4-16-2014.JPG]]<br /> <br /> PCR from 80 pLex-p53wt&lt;br&gt;p53-wt-BamH1-F &lt;br&gt;p53-wt-EcoR1-R&lt;br&gt;<br /> <br /> [[Image:2141_2142_2143_2168clone_4-16-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;231&quot; height=&quot;82&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1973 pCMV5-CD16-RIgE&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | BamH1 + EcoR1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-23<br /> <br /> Make miniprep and digest with BamH1 + EcoR1&lt;br&gt;<br /> <br /> [[Image:2141_2142digest_4-23-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; [[Category:Cloning]]</div> Paul.xu