https://eln.excellgen.com/index.php?action=history&feed=atom&title=2145-pT7A3HC-6H-Tat-MS2 2026-10-18T12:00:03Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2145-pT7A3HC-6H-Tat-MS2&diff=11651&oldid=prev 2014-04-09T18:31:42Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2145-pT7A3HC-6H-Tat-MS2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2145-pT7A3HC-6H-Tat-MS2<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-6---2014-4-9<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2145&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:732_2145_2147_2152_2155_2174_2180PCR_4-7-2014.JPG]]<br /> <br /> Use MS2-1 + MS2-linker-R to amplify oligo mixture of MS2-1 to MS2-12&lt;br&gt;&lt;br&gt;<br /> <br /> [[Image:732_1841_2145_2147clone_4-7-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR&lt;br&gt;<br /> | 1885 pT7A3-HC&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nco1 + BamH1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-9 <br /> <br /> Make miniprep and digest with Nco1 + BamH1&lt;br&gt; <br /> <br /> [[Image:2145 2147digest 4-9-2014.JPG]]&lt;br&gt; <br /> <br /> c:vector<br /> <br /> &amp;lt;br&amp;gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; [[Category:Cloning]]</div>

Paul.xu