https://eln.excellgen.com/index.php?action=history&feed=atom&title=2146-pT7A3HC-6H-Tat-sc-MS2 2146-pT7A3HC-6H-Tat-sc-MS2 - Revision history 2026-10-18T10:50:48Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2146-pT7A3HC-6H-Tat-sc-MS2&diff=11712&oldid=prev Paul.xu at 19:19, 17 April 2014 2014-04-17T19:19:42Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2146-pT7A3HC-6H-Tat-sc-MS2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2146-pT7A3HC-6H-Tat-sc-MS2 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-14---2014 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2146&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2146pcr1 4-14-2014.JPG]][[Image:2148pcr2 4-3-2014.JPG]]&lt;br&gt; <br /> <br /> pcr1 MS2 fragment to amplify MS2 from 2145 pT7A3HC-6H-Tat-MS2&lt;br&gt;MS2-linker-F &lt;br&gt;MS2-EcoR1-Term-R &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> pcr2: same with 2148pcr2&lt;br&gt; <br /> <br /> T7 promoter from 706 pRsetA &lt;br&gt;Term-T7-F&lt;br&gt;T7-HindIII-R&lt;br&gt; <br /> <br /> <br /> <br /> 2014-4-17<br /> <br /> [[Image:2146PCR 4-16-2014.JPG]]&lt;br&gt; <br /> <br /> PCR:pcr1+pcr2&lt;br&gt; <br /> <br /> MS2-linker-F &lt;br&gt;T7-HindIII-R &lt;br&gt; <br /> <br /> [[Image:2146clone 4-17-2014.JPG]] <br /> <br /> {| width=&quot;300&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR&lt;br&gt; <br /> | PCR&lt;br&gt; <br /> | 2145, pT7A3HC-6H-Tat-MS2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;3&quot; | BamH1 + HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-17 <br /> <br /> Make miniprep and digest with&amp;nbsp; BamH1+Hind111&lt;br&gt; <br /> <br /> [[Image:2146digest1 4-17-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu